Coding

Part:BBa_K3384315:Design

Designed by: Yiqing Zhang   Group: iGEM20_NJTech_China   (2020-10-26)

Ste5ΔN-CTM


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 365
    Illegal SpeI site found at 1620
    Illegal SpeI site found at 2166
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 365
    Illegal SpeI site found at 1620
    Illegal SpeI site found at 2166
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 365
    Illegal BglII site found at 813
    Illegal BglII site found at 2121
    Illegal XhoI site found at 2058
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 365
    Illegal SpeI site found at 1620
    Illegal SpeI site found at 2166
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 365
    Illegal SpeI site found at 1620
    Illegal SpeI site found at 2166
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Ste5ΔN (N-terminal truncated 214aa Ste5 protein coding sequence) and CTM (The coding sequence of the C-terminal transmembrane domain of Snc2 protein) were obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as the template.

Source

Saccharomyces cerevisiae

References

Lamson, R. E., Takahashi, S., Winters, M. J., and Pryciak, P. M. (2006) Dual role for membrane localization in yeast MAP kinase cascade activation and its contribution to signaling fidelity, Curr Biol 16, 618-623.